Difference between revisions of "Team:HK SSC"

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                        HK_SSC</a></span>
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                        Home</a>
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                        Team<br></a>
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<section class="engine"><a href="https://mobirise.info/s">bootstrap themes</a></section><section class="header1 cid-rAVtEy3FNI" id="header16-1">
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* Docs to Markdown version 1.0β17
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* Wed Sep 25 2019 09:54:22 GMT-0700 (PDT)
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* Source doc: https://docs.google.com/open?id=1cax56EvETOwJh0Be8TEAk4eDMd43HW1cMOFAlzyRcpw
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* This is a partial selection. Check to make sure intra-doc links work.
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<p>Project description
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<h3>Abstract/ Overview</h3>
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Microcystis aeruginosa is one of the most common cyanobacteria responsible for
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harmful algal blooms. This cyanobacterium produces microcystin, a hepatotoxin
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that damages the liver. However, direct lysis of Microcystis aeruginosa may not
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                <h1 class="mbr-section-title mbr-bold pb-3 mbr-fonts-style display-1">
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best for the environment as it holds ecological values of heavy metal sorption
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                    ABSTRACT</h1>
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and oxygen synthesis. In this project, we hope to silence the microcystin
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biosynthesis cluster(mcy) using a catalytically dead Cas9 (dCas9) enzyme lacking
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endonuclease activity. When the dCas9 enzyme is co-expressed with a guide
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RNA(sgRNA), the dCas9-sgRNA complex specifically binds to the McyB gene and
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blocks transcript elongation, leading to the repression of the McyB gene without
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altering the chromosome of the Microcystis. Here we provide the design of a
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dCas9-sgRNA expression gene in a shuttle vector that can replicate in both
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E.coli and cyanobacteria. We will also be conducting downstream analysis to see
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how our dCas9-sgRNA expression plasmid affects the microcystin-production rate
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and oxygen synthesis rate of Microcystis.
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                    Project Inspiration</h1>
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                    Our project is inspired from various aspects, including school lessons, books, news articles hiking trips, and even previous iGEM teams.
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Revision as of 16:54, 25 September 2019


Project description

Abstract/ Overview

Microcystis aeruginosa is one of the most common cyanobacteria responsible for harmful algal blooms. This cyanobacterium produces microcystin, a hepatotoxin that damages the liver. However, direct lysis of Microcystis aeruginosa may not best for the environment as it holds ecological values of heavy metal sorption and oxygen synthesis. In this project, we hope to silence the microcystin biosynthesis cluster(mcy) using a catalytically dead Cas9 (dCas9) enzyme lacking endonuclease activity. When the dCas9 enzyme is co-expressed with a guide RNA(sgRNA), the dCas9-sgRNA complex specifically binds to the McyB gene and blocks transcript elongation, leading to the repression of the McyB gene without altering the chromosome of the Microcystis. Here we provide the design of a dCas9-sgRNA expression gene in a shuttle vector that can replicate in both E.coli and cyanobacteria. We will also be conducting downstream analysis to see how our dCas9-sgRNA expression plasmid affects the microcystin-production rate and oxygen synthesis rate of Microcystis.