Team:XJTU-CHINA/Parts
Parts
Basic & Composite Parts
| Part Name | Basic/Composite | Number | Description |
|---|---|---|---|
| (4S)-limonene synthase | Basic | BBa_K3052001 | Convert GPP to limonene |
| (S)-linalool synthase: LGF | Basic | BBa_K3052002 | Convert GPP to linalool (alternatives 1) |
| (S)-linalool synthase: RXY | Basic | BBa_K3052003 | Convert GPP to linalool (alternatives 2) |
| (S)-linalool synthase: DHJ | Basic | BBa_K3052004 | Convert GPP to linalool (alternatives 3) |
| Precursor Circuit | Basic | BBa_K3052010 | a series of enzymes used for producing GPP |
| Ptrc+limonene synthase+bacterial terminator | Basic | BBa_K3052012 | Production circuit for validation of limonene synthase |
| Ptrc+linalool synthase(LGF)+bacterial terminator | Basic | BBa_K3052013 | Production circuit for validation of linalool synthase (LGF) |
| Ptrc+linalool synthase(RXY)+bacterial terminator | Basic | BBa_K3052014 | Production circuit for validation of linalool synthase (RXY) |
| Ptrc+linalool synthase(DHJ)+bacterial terminator | Basic | BBa_K3052015 | Production circuit for validation of linalool synthase (DHJ) |
| light controlling circuit - LGF | Composite | BBa_K3052021 | Red light control system enabling selective production of limonene and linalool |
| light controlling circuit - RXY | Composite | BBa_K3052022 | Red light control system enabling selective production of limonene and linalool |
| light controlling circuit - DHJ | Composite | BBa_K3052023 | Red light control system enabling selective production of limonene and linalool |
| Light-fluoresce circuit (GFP&RFP) | Composite | BBa_K3052030 | Light-fluorescent circuit for validation of light-controlling circuit by measuring the intensity of sfGFP and mRFP |
| Light-fluoresce circuit (GFP)- LGF | Composite | BBa_K3052031 | Light-fluorescent circuit for validation of light-controlling circuit by measuring the intensity of sfGFP |
| Light-fluoresce circuit (GFP)- RXY | Composite | BBa_K3052032 | Light-fluorescent circuit for validation of light-controlling circuit by measuring the intensity of sfGFP |
| Light-fluoresce circuit (GFP)- DHJ | Composite | BBa_K3052033 | Light-fluorescent circuit for validation of light-controlling circuit by measuring the intensity of sfGFP |
| Light-fluoresce circuit (RFP) - CS | Composite | BBa_K3052034 | Light-fluorescent circuit for validation of light-controlling circuit by measuring the intensity of mRFP |
| sfGFP | Basic | BBa_K3052041 | Superfolder green fluorescent protein |
| mRFP | Basic | BBa_K3052042 | m1 red fluorescent protein |
| Cph8-pompC-sfGFP | Composite | BBa_K3052054 | This part is the composite product of Golden Gate Assembly of our light-controlling circuit. |
| Cph8-pompC-LS | Composite | BBa_K3052055 | This part is the composite product of Golden Gate Assembly of our light-controlling circuit. |
| CI+Pλ+mRFP+ho1-pcyA | Composite | BBa_K3052056 | This part is the composite product of Golden Gate Assembly of our light-controlling circuit. |
| CI+Pλ+LGF+ho1-pcyA | Composite | BBa_K3052057 | This part is the composite product of Golden Gate Assembly of our light-controlling circuit. |
| CI+Pλ+RXY+ho1-pcyA | Composite | BBa_K3052058 | This part is the composite product of Golden Gate Assembly of our light-controlling circuit. |
| CI+Pλ+DHJ+ho1-pcyA | Composite | BBa_K3052059 | This part is the composite product of Golden Gate Assembly of our light-controlling circuit. |
Part Collection
Overview
A light control system forms a twp-phases switch can be used to control the production of limonene and linalool by only changing physical conditions. The central part of this light control system is a photosensitive protein --Cph8. When the environment is dark, this protein will be normally activated and binds with OmpR, the formed complex can activate the expression of the downstream genes, produces CS and a thermosensitive inhibitor CI that can block the expression of gene regulated by pλ, which is LS here. CI responses to changes in temperature, specifically it will be deactivated when temperature rises to 42 degrees, thereby LS expresses again. With the presence of red light, Cph8 will be deactivated and expression of CS and CI will terminate.
Generally, the red light in our device will be shut down at night, which means linalool will be synthesized for a nice sleep while limonene will start to be produced with red light in the morning to remove sleepy mind.
Furthermore, we have constructed several light-fluorescent circuits wherein the CS and LS are substituted by superfolder GFP and m1RFP respectively. The peak value and of the two phases and responding time of the switch can be calculated by measuring the fluoresce intensity. In order not to cause the interference between GFP and RFP, they are also placed on two different circuits.
| Part Name | Basic/Composite | Number | Description |
|---|---|---|---|
| light controlling circuit - LGF | Composite | BBa_K3052021 | Red light control system enabling selective production of limonene and linalool |
| light controlling circuit - RXY | Composite | BBa_K3052022 | Red light control system enabling selective production of limonene and linalool |
| light controlling circuit - DHJ | Composite | BBa_K3052023 | Red light control system enabling selective production of limonene and linalool |
| Light-fluoresce circuit (GFP&RFP) | Composite | BBa_K3052030 | Light-fluorescent circuit for validation of light-controlling circuit by measuring the intensity of sfGFP and mRFP |
| Light-fluoresce circuit (GFP)- LGF | Composite | BBa_K3052031 | Light-fluorescent circuit for validation of light-controlling circuit by measuring the intensity of sfGFP |
| Light-fluoresce circuit (GFP)- RXY | Composite | BBa_K3052032 | Light-fluorescent circuit for validation of light-controlling circuit by measuring the intensity of sfGFP |
| Light-fluoresce circuit (GFP)- DHJ | Composite | BBa_K3052033 | Light-fluorescent circuit for validation of light-controlling circuit by measuring the intensity of sfGFP |
| Light-fluoresce circuit (RFP) - CS | Composite | BBa_K3052034 | Light-fluorescent circuit for validation of light-controlling circuit by measuring the intensity of mRFP |
Part Table
Please include a table of all the parts your team has made during your project on this page. Remember part characterization and measurement data must go on your team part pages on the Registry.
<groupparts>iGEM19 XJTU-CHINA</groupparts>