Attributions
Project Design
All the students participated in brainstorming. We picked the two most interesting ideas - cell radio and bacterial fragrance generator. At last, we voted to optimize one of the programs, and Wenhe Ma and Jun Yang made the final design.
Dry Lab
We have modeled our project from many aspects.
Model design
Chenxing Qian, Jin Ning, Jiankai Chang
Equation analysis
Jin Ning
Parameter search and detection
Chenxing Qian, Yihui Wan, Weizhe Wang
Coding & debugging
Chenxing Qian, Yilun Liu
Wet Lab
Our wet experiments can be divided into four main areas: plasmid construction, expression level detection, product detection and fluorescence detection. Therefore, we have a division of labor and cooperation.
Precursor circuit construction
- Design: Wenhe Ma, Jun Yang
- Molecules Clonings: Mengmeng Xu, Wenhao wang
Production circuit construction
- Design: Wenhe Ma, Jun Yang
- Molecules Clonings: Mengmeng Xu, Wenhao Wang, Jialu Li
Controlling circuit construction
- Design: Wenhe Ma, Jun Yang, Mengmeng Xu
- Molecules Clonings: Wenhao Wang, Jialu Li
Fluorescent circuit construction
- Design: Wenhe Ma, Jun Yang
- Molecules clonings: Wenhao Wang, Jialu Li
Expression level measurement
- Design: Mengmeng Xu
- SDS page: Wenhao Wang, Weizhe Wang
- RT-PCR: Mengmeng Xu, Yang Zhang
Product detection
- Design: Chenxing Qian, Jiaxin He
- Gas Chromatography: Jiaxin He, Jun Yang
Fluorescence detection
- Design: Mengmeng Xu, Jun Yang
- Fluorescence detection: Mengmeng Xu, Jun Yang, Yunyi Miao, Siyue Fan
- Growth curve: Wenhao Wang, Jialu Li
Others (Bacterial picture)
- Fluorescence microscope: Jun Yang
- Laser scanning confocal microscope: Mengmeng Xu
Hardware
We have designed a matching hardware for our project.
Design
Jinwei Xu, Wenhe Ma
Software
Jinwei Xu, Guofan Fan
3D printing
Jinwei Xu, Longning Huang
Human Practice
We have done plenty of activities for Human Practice. Almost all of us have participated in this part.
Meet up
- May 5th Xi Nan Meet up:
Wenhe Ma, Mengmeng Xu, Jinwei Xu, Chenxing Qian - July 19th Shannxi Meet up:
All of the team - August 19th-August 23th:
Mengmeng Xu, Jun Yang, Jialu Li
Integrated HP
- Interview with Prof. Wei Dang:
Yang Zhang, Yunyi Miao, Mengmeng Xu, Yongjing Li - Interview with Prof. Yali Zhang:
Mengmeng Xu, Yang Zhang, Jinwei Xu, Jun Yang, Wenhe Ma - Interview with Prof. Jin Xu:
Yang Zhang, Yunyi Miao, Mengmeng Xu - Interview with Prof. Jiangang Long:
Yang Zhang, Yunyi Miao, Yihui Wan, Weizhe Wang, Siyue Fan - Yuansen Biological Techology Co., Ltd:
Shuhui Zhang, Yang Zhang, Wenhe Ma, Jiankai Chang - Xi'an Natural Field Bio-technique Co., Ltd:
Shuhui Zhang, Wenhe Ma, Jiankai Chang, Yihui Wan, Jialu Li
Social media
Wechat account: Yunyi Miao, Yang Zhang, Mengmeng Xu
Public engagement
- Sun Village Tour: All of the team
- Science camp activity: All of the team
- Visit the Instrument Analysis Test Sharing Center: All of the team
- Sleep quality survey: Yunyi Miao, Mengmeng Xu
- Survey on hardware design: Yang Zhang, Yunyi Miao, Jinwei Xu
Wiki Construction
Yilun Liu, Wenhe Ma
Art Design
- Overall design: Xiaotong Sui
- Poster design: Yilun Liu, Jie Shao
- Video clip: Daoyuan Yang, Weizhe Wang
Advisors
IGEM is not a one-time journey. After the IGEM competition, we have more time for activities such as After-IGEM etc. We appreciate three IGEMers from last year's iGEM team for helping us a lot. In addition, we are also very grateful to the senior students guiding us on our experiments.
Yujia Liu
When we were brainstorming, Yujia Liu gave us a lot of useful suggestions and taught us to consult the literature. Additionally, he introduced us a variety of methods for constructing plasmids which has a profound impact on our project design.
Xinlei Fang
As a major member of the iGEM experimental group last year, Xinlei Fang told us a lot of experimental tips and experimental precautions.
Jiabao Li
In the process of project advance, Jiabao Li always patiently reminded us of the deadlines for various matters. Besides, he also helped us in modeling.
Jiping Zhao
Jiping Zhao, phD student, has rich experience in experiments. She taught us a lot experimental skills and guided us to do primer design and plasmid design.
External Assistance
IGEM is a long journey. In the process of moving forward, we have met many intelligent and dynamic partners. We are very grateful for their kind help.
Yongjing Li
Yongjing Li helped us a lot in Human practice. She did two wonderful interviews for our team and got a lot of information from experts on the development of our project. As an outgoing and kind person, she was always active in education and public engagement.
Qihan Meng
When we firstly established our project, Qihan Meng helped us to do research on sleep quality and problems. Meanwhile, she also helped us to analyze our data and consult relevant research data in the literature.
Longning Huang
Longning Huang provided us with aerial photography equipment and took group photos of our team. When we have problems with hardware design, he also provided 3D printing help for us.
Bo Diao
Bo Diao helped us to design our hardware and search for relevant modeling parameters.
Guofan Fan
Guofan Fan helped to construct our APP coupling with the hardware.
PIs & Instructors
IGEM is also a rare research experience, we are very grateful to our PIs and Instructors for their patient assistance.
Xiaoyun Lu
Professor Lu encouraged us to carry out innovative practices. She provided us with good experimental conditions. With her suggestion, we have chosen fragrance molecules which can improve sleep conditions. She also provided us with great ideas to make our project more attractive.
Dan Tan
Dr.Tan gave us detailed information on our experiments. She taught us to construct plasmid maps and optimize our experimental schedule. She suggested that we perform separate characterization of the parts.
Shuhong Sun
Teacher Sun helped us in our experiments and taught us about lab safety.
Shuyan Qi
Teacher Qi taught us a lot of experimental skills.
Thank you for your company and guidance along the way!
Acknowledgment
Financial and Facilities Support
Jiankang LIU, PhD. Professor
Dean of College of Life Science and Technology, Xi'an Jiaotong University
Jianbao ZHANG, PhD, Professor
Associate dean of College of Life Science and Technology, Xi'an Jiaotong University
Xi'an Jiaotong University
School of Life Science and Technology, Xi'an Jiaotong University
Qian Xuesen College, Xi'an Jiaotong University
School of Science, Xi'an Jiaotong University
Instrumental Analysis Sharing Center, Xi'an Jiaotong University
Project Support
People
Jiankang LIU, PhD. Professor
Dean of College of Life Science and Technology, Xi’an Jiaotong University
Gave suggestions and support for our project.
Jiangang LONG, PhD, Professor
Associate dean of College of Life Science and Technology, Xi’an Jiaotong University
Gave suggestions and support for our project.
Yali ZHANG, PhD, Professor
Professor of School of Life Science and Technology, Xi’an Jiaotong University
Helped us a lot in our hardware design.
Jin XU, PhD, Professor
Deputy Director of Institute of Biomedical Engineering, Xi'an Jiaotong University School of Life
Gave us information of sleep problem and its detection method.
Changhe WANG, PhD, Professor
Professor of School of Life Science and Technology, Xi’an Jiaotong University
Give us support for Human Practice activities and Facilities Support.
Huadong LIU, PhD, Professor
Professor of School of Life Science and Technology, Xi’an Jiaotong University
Give us support for Human Practice activities.
Wei DANG, PhD
Deputy Chief Physician, Xi'an Mental Health Research Center
Gave us advice on Sleep problem treatment.
Shangwei LV, PhD
An expert in the transformation of knowledge into practice
Gave us advice about entrepreneurship.
Xiang JIN
CEO of Yesherb company
Had an interview with us and provide information of Aromatherapy products.
Companies & Organizations
BluePHA (Beijing) Co., Ltd.
Provided PJEB6409 Plasmid for us.
Shaanxi Bio-Agriculture Institute, Microbiology Institute of Shaanxi
Provided advices about fermentation culture of bacteria.
Xi’an Natural Field Bio-technique (Xi'an) Co., Ltd.
Company visit and gave us advices.
Xi´an Yuensun Biological Technology Co., Ltd.
Company visit and gave us advices.
Sun Village Institution (NGO, Xi’an)
Gave us assistance and support for Human Practice activities for the special children.
Teams
NWU-China
Provided pSb1k3 plasmid for us and helped us with modeling part.
YAU-China
Provided pSb1k3 plasmid for us.
GreatBay-China (2018)
Provided PJEB6409 plasmid for us and shared experience with us.
UCAS-China (2018)
Provided light-switch related parts for us.
University of Electronic Science and Technology of China
Held the Xi Nan Meetup and shared experience with us.
Third Military Medical University
Gave advice for our project and shared experience with us.
iGEM Competition Related Support
Daocheng WU, PhD, Professor
Professor in Xi’an Jiaotong University
Huadong LIU, PhD, Professor
Professor in Xi’an Jiaotong University
Jian DING,PhD, Professor
Professor in Xi’an Jiaotong University
Changhe WANG, PhD, Professor
Professor in Xi’an Jiaotong University
Miss Meagan LIZARAZO
Vice President of iGEM
Terry JOHNSON, Professor
Professor in Biological Engineering, judge in iGEM
Li CHENG
PhD Candidate, Peking University, Volunteer in CCiC
Haoqian ZHANG
CEO of Bulepha Co., Ltd. previous advisor of Peking-China team
Nan ZHANG
Master Candidate, University of Edinburgh, Asian ambassador of IGEM official