Team:XJTU-CHINA/Attributions

Attributions

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Project Design

All the students participated in brainstorming. We picked the two most interesting ideas - cell radio and bacterial fragrance generator. At last, we voted to optimize one of the programs, and Wenhe Ma and Jun Yang made the final design.

Dry Lab

We have modeled our project from many aspects.

Model design

Chenxing Qian, Jin Ning, Jiankai Chang

Equation analysis

Jin Ning

Parameter search and detection

Chenxing Qian, Yihui Wan, Weizhe Wang

Coding & debugging

Chenxing Qian, Yilun Liu

Wet Lab

Our wet experiments can be divided into four main areas: plasmid construction, expression level detection, product detection and fluorescence detection. Therefore, we have a division of labor and cooperation.

Precursor circuit construction

  • Design: Wenhe Ma, Jun Yang
  • Molecules Clonings: Mengmeng Xu, Wenhao wang

Production circuit construction

  • Design: Wenhe Ma, Jun Yang
  • Molecules Clonings: Mengmeng Xu, Wenhao Wang, Jialu Li

Controlling circuit construction

  • Design: Wenhe Ma, Jun Yang, Mengmeng Xu
  • Molecules Clonings: Wenhao Wang, Jialu Li

Fluorescent circuit construction

  • Design: Wenhe Ma, Jun Yang
  • Molecules clonings: Wenhao Wang, Jialu Li

Expression level measurement

  • Design: Mengmeng Xu
  • SDS page: Wenhao Wang, Weizhe Wang
  • RT-PCR: Mengmeng Xu, Yang Zhang

Product detection

  • Design: Chenxing Qian, Jiaxin He
  • Gas Chromatography: Jiaxin He, Jun Yang

Fluorescence detection

  • Design: Mengmeng Xu, Jun Yang
  • Fluorescence detection: Mengmeng Xu, Jun Yang, Yunyi Miao, Siyue Fan
  • Growth curve: Wenhao Wang, Jialu Li

Others (Bacterial picture)

  • Fluorescence microscope: Jun Yang
  • Laser scanning confocal microscope: Mengmeng Xu

Hardware

We have designed a matching hardware for our project.

Design

Jinwei Xu, Wenhe Ma

Software

Jinwei Xu, Guofan Fan

3D printing

Jinwei Xu, Longning Huang

Human Practice

We have done plenty of activities for Human Practice. Almost all of us have participated in this part.

Meet up

  • May 5th Xi Nan Meet up:
    Wenhe Ma, Mengmeng Xu, Jinwei Xu, Chenxing Qian
  • July 19th Shannxi Meet up:
    All of the team
  • August 19th-August 23th:
    Mengmeng Xu, Jun Yang, Jialu Li

Integrated HP

  • Interview with Prof. Wei Dang:
    Yang Zhang, Yunyi Miao, Mengmeng Xu, Yongjing Li
  • Interview with Prof. Yali Zhang:
    Mengmeng Xu, Yang Zhang, Jinwei Xu, Jun Yang, Wenhe Ma
  • Interview with Prof. Jin Xu:
    Yang Zhang, Yunyi Miao, Mengmeng Xu
  • Interview with Prof. Jiangang Long:
    Yang Zhang, Yunyi Miao, Yihui Wan, Weizhe Wang, Siyue Fan
  • Yuansen Biological Techology Co., Ltd:
    Shuhui Zhang, Yang Zhang, Wenhe Ma, Jiankai Chang
  • Xi'an Natural Field Bio-technique Co., Ltd:
    Shuhui Zhang, Wenhe Ma, Jiankai Chang, Yihui Wan, Jialu Li

Social media

Wechat account: Yunyi Miao, Yang Zhang, Mengmeng Xu

Public engagement

  • Sun Village Tour: All of the team
  • Science camp activity: All of the team
  • Visit the Instrument Analysis Test Sharing Center: All of the team
  • Sleep quality survey: Yunyi Miao, Mengmeng Xu
  • Survey on hardware design: Yang Zhang, Yunyi Miao, Jinwei Xu

Wiki Construction

Yilun Liu, Wenhe Ma

Art Design

  • Overall design: Xiaotong Sui
  • Poster design: Yilun Liu, Jie Shao
  • Video clip: Daoyuan Yang, Weizhe Wang

Advisors

IGEM is not a one-time journey. After the IGEM competition, we have more time for activities such as After-IGEM etc. We appreciate three IGEMers from last year's iGEM team for helping us a lot. In addition, we are also very grateful to the senior students guiding us on our experiments.

Yujia Liu

When we were brainstorming, Yujia Liu gave us a lot of useful suggestions and taught us to consult the literature. Additionally, he introduced us a variety of methods for constructing plasmids which has a profound impact on our project design.

Xinlei Fang

As a major member of the iGEM experimental group last year, Xinlei Fang told us a lot of experimental tips and experimental precautions.

Jiabao Li

In the process of project advance, Jiabao Li always patiently reminded us of the deadlines for various matters. Besides, he also helped us in modeling.

Jiping Zhao

Jiping Zhao, phD student, has rich experience in experiments. She taught us a lot experimental skills and guided us to do primer design and plasmid design.

External Assistance

IGEM is a long journey. In the process of moving forward, we have met many intelligent and dynamic partners. We are very grateful for their kind help.

Yongjing Li

Yongjing Li helped us a lot in Human practice. She did two wonderful interviews for our team and got a lot of information from experts on the development of our project. As an outgoing and kind person, she was always active in education and public engagement.

Qihan Meng

When we firstly established our project, Qihan Meng helped us to do research on sleep quality and problems. Meanwhile, she also helped us to analyze our data and consult relevant research data in the literature.

Longning Huang

Longning Huang provided us with aerial photography equipment and took group photos of our team. When we have problems with hardware design, he also provided 3D printing help for us.

Bo Diao

Bo Diao helped us to design our hardware and search for relevant modeling parameters.

Guofan Fan

Guofan Fan helped to construct our APP coupling with the hardware.

PIs & Instructors

IGEM is also a rare research experience, we are very grateful to our PIs and Instructors for their patient assistance.

Xiaoyun Lu

Professor Lu encouraged us to carry out innovative practices. She provided us with good experimental conditions. With her suggestion, we have chosen fragrance molecules which can improve sleep conditions. She also provided us with great ideas to make our project more attractive.

Dan Tan

Dr.Tan gave us detailed information on our experiments. She taught us to construct plasmid maps and optimize our experimental schedule. She suggested that we perform separate characterization of the parts.

Shuhong Sun

Teacher Sun helped us in our experiments and taught us about lab safety.

Shuyan Qi

Teacher Qi taught us a lot of experimental skills.

Thank you for your company and guidance along the way!

Acknowledgment

Financial and Facilities Support

Jiankang LIU, PhD. Professor

Dean of College of Life Science and Technology, Xi'an Jiaotong University

Jianbao ZHANG, PhD, Professor

Associate dean of College of Life Science and Technology, Xi'an Jiaotong University

Xi'an Jiaotong University

School of Life Science and Technology, Xi'an Jiaotong University

Qian Xuesen College, Xi'an Jiaotong University

School of Science, Xi'an Jiaotong University

Instrumental Analysis Sharing Center, Xi'an Jiaotong University

Project Support

People

Jiankang LIU, PhD. Professor

Dean of College of Life Science and Technology, Xi’an Jiaotong University

Gave suggestions and support for our project.

Jiangang LONG, PhD, Professor

Associate dean of College of Life Science and Technology, Xi’an Jiaotong University

Gave suggestions and support for our project.

Yali ZHANG, PhD, Professor

Professor of School of Life Science and Technology, Xi’an Jiaotong University

Helped us a lot in our hardware design.

Jin XU, PhD, Professor

Deputy Director of Institute of Biomedical Engineering, Xi'an Jiaotong University School of Life

Gave us information of sleep problem and its detection method.

Changhe WANG, PhD, Professor

Professor of School of Life Science and Technology, Xi’an Jiaotong University

Give us support for Human Practice activities and Facilities Support.

Huadong LIU, PhD, Professor

Professor of School of Life Science and Technology, Xi’an Jiaotong University

Give us support for Human Practice activities.

Wei DANG, PhD

Deputy Chief Physician, Xi'an Mental Health Research Center

Gave us advice on Sleep problem treatment.

Shangwei LV, PhD

An expert in the transformation of knowledge into practice

Gave us advice about entrepreneurship.

Xiang JIN

CEO of Yesherb company

Had an interview with us and provide information of Aromatherapy products.

Companies & Organizations

BluePHA (Beijing) Co., Ltd.

Provided PJEB6409 Plasmid for us.

Shaanxi Bio-Agriculture Institute, Microbiology Institute of Shaanxi

Provided advices about fermentation culture of bacteria.

Xi’an Natural Field Bio-technique (Xi'an) Co., Ltd.

Company visit and gave us advices.

Xi´an Yuensun Biological Technology Co., Ltd.

Company visit and gave us advices.

Sun Village Institution (NGO, Xi’an)

Gave us assistance and support for Human Practice activities for the special children.

Teams

NWU-China

Provided pSb1k3 plasmid for us and helped us with modeling part.

YAU-China

Provided pSb1k3 plasmid for us.

GreatBay-China (2018)

Provided PJEB6409 plasmid for us and shared experience with us.

UCAS-China (2018)

Provided light-switch related parts for us.

University of Electronic Science and Technology of China

Held the Xi Nan Meetup and shared experience with us.

Third Military Medical University

Gave advice for our project and shared experience with us.

iGEM Competition Related Support

Daocheng WU, PhD, Professor

Professor in Xi’an Jiaotong University

Huadong LIU, PhD, Professor

Professor in Xi’an Jiaotong University

Jian DING,PhD, Professor

Professor in Xi’an Jiaotong University

Changhe WANG, PhD, Professor

Professor in Xi’an Jiaotong University

Miss Meagan LIZARAZO

Vice President of iGEM

Terry JOHNSON, Professor

Professor in Biological Engineering, judge in iGEM

Li CHENG

PhD Candidate, Peking University, Volunteer in CCiC

Haoqian ZHANG

CEO of Bulepha Co., Ltd. previous advisor of Peking-China team

Nan ZHANG

Master Candidate, University of Edinburgh, Asian ambassador of IGEM official

Thanks for all of us involved!