Team:WLC-Milwaukee/Improve
Parts
Characterized Parts
BBa_K1900000 and BBa_K 1900001
This summer, the wet lab team also did work with previous parts from WLC-Milwaukee (BBa_K1900000 and BBa_K 1900001). In this work, the tolC genes from Klebsiella pneumoniae and Serratia marcescens were cloned into a plasmid containing the pTrc promoter. This plasmid was then transformed into an E. coli strain that lacked a chromosomal copy of tolC. We then performed disk sensitivity assays to determine how well the S. marcescens and K. pneumoniae TolC protein could perform in an E. Coli cell. Please see the results section for more information on these experiments.
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- star_borderWe improved the characterization of parts BBa_K1900000 and BBa_K1900001 by providing more data regarding the tolC protein and its role in antibiotic resistance.
