Notebook

Lab Journal Experiments

Parts

Overview Basic Parts

Team

Team members Attributions Achievements Collaborations

Human Practices

Human Practices Pathogens Detections Our Approach Public Engagement Expert Insight Market Consultation Safety

Project

Description Design Characterization Results Model Software Hardware

Parts head

Parts Overview

The parts that we designed for this project are all ToeHold switches used to detect specific target sequences. Initially, we designed them to target easily accessible E. coli gene, and the ToeHolds were designed by hand. They were cloned in expression plasmids and tested in cell-free expression system, myTXTL by ArborBiosciences.

Parts that we designed to target viral sequences were designed using our software tool. However, we did not have time to test them using viral nucleic acid extract. All switches were designed using sequence constraint and common sequences from the most efficient switches from the Green et al. 2014 paper from Cell, which introduced synthetic ToeHolds switches.

**Figure 1.** SBOL map of the ToeHold switches in vector pNZ123. All ToeHold switches designed for this project were designed following this pattern. Only ToeHold sequence varies between switches. pNZ123 was selected because of it high-copy number, small size and versatile replication origin.

List of Basic Parts
Name	Type	Description	Designer	Length
BBa_K3026000	Reporter	ToeHold_DNA_AmpR	Francois D Rouleau	1002
BBa_K3026001	Reporter	ToeHold_DNA_AmpR	Francois D Rouleau	993
BBa_K3026002	Reporter	ToeHold_DNA_FtsK	Francois D Rouleau	1002
BBa_K3026003	Reporter	ToeHold_DNA_FtsK	Francois D Rouleau	993
BBa_K3026004	Reporter	ToeHold_DNA_SecA	Francois D Rouleau	1002
BBa_K3026005	Reporter	ToeHold_DNA_SecA	Francois D Rouleau	993
BBa_K3026006	Reporter	ToeHold_DNA_LacZ	Francois D Rouleau	1002
BBa_K3026007	Reporter	ToeHold_DNA_repOri	Francois D Rouleau	1002
BBa_K3026008	Reporter	ToeHold_DNA_MATalpha	Francois D Rouleau	997
BBa_K3026010	Reporter	Toehold norovirus ORF2 VP1	Pascale Lemieux	1001
BBa_K3026011	Reporter	Toehold norovirus ORF2 VP1	Pascale Lemieux	1001
BBa_K3026012	Reporter	Toehold norovirus ORF2 VP1	Pascale Lemieux	1001
BBa_K3026013	Reporter	Toehold measles virus MG912594.1	Pascale Lemieux	1001
BBa_K3026014	Reporter	Toehold measles virus MG912594.1	Pascale Lemieux	1001
BBa_K3026015	Reporter	Toehold measles virus MG912594.1	Pascale Lemieux	1001
BBa_K3026016	Reporter	Toehold chickenpox virus BAC44882.1	Pascale Lemieux	1001
BBa_K3026017	Reporter	Toehold chickenpox virus BAC44882.1	Pascale Lemieux	1001
BBa_K3026018	Reporter	Toehold chickenpox virus BAC44882.1	Pascale Lemieux	1001