Team:ULaval/Parts

Team:ULaval - 2019.igem.org


Parts head

Parts Overview

The parts that we designed for this project are all ToeHold switches used to detect specific target sequences. Initially, we designed them to target easily accessible E. coli gene, and the ToeHolds were designed by hand. They were cloned in expression plasmids and tested in cell-free expression system, myTXTL by ArborBiosciences.

Parts that we designed to target viral sequences were designed using our software tool. However, we did not have time to test them using viral nucleic acid extract. All switches were designed using sequence constraint and common sequences from the most efficient switches from the Green et al. 2014 paper from Cell, which introduced synthetic ToeHolds switches.




Figure 1. SBOL map of the ToeHold switches in vector pNZ123. All ToeHold switches designed for this project were designed following this pattern. Only ToeHold sequence varies between switches. pNZ123 was selected because of it high-copy number, small size and versatile replication origin.



List of Basic Parts

Name Type Description Designer Length
BBa_K3026000 Reporter ToeHold_DNA_AmpR Francois D Rouleau 1002
BBa_K3026001 Reporter ToeHold_DNA_AmpR Francois D Rouleau 993
BBa_K3026002 Reporter ToeHold_DNA_FtsK Francois D Rouleau 1002
BBa_K3026003 Reporter ToeHold_DNA_FtsK Francois D Rouleau 993
BBa_K3026004 Reporter ToeHold_DNA_SecA Francois D Rouleau 1002
BBa_K3026005 Reporter ToeHold_DNA_SecA Francois D Rouleau 993
BBa_K3026006 Reporter ToeHold_DNA_LacZ Francois D Rouleau 1002
BBa_K3026007 Reporter ToeHold_DNA_repOri Francois D Rouleau 1002
BBa_K3026008 Reporter ToeHold_DNA_MATalpha Francois D Rouleau 997
BBa_K3026010 Reporter Toehold norovirus ORF2 VP1 Pascale Lemieux 1001
BBa_K3026011 Reporter Toehold norovirus ORF2 VP1 Pascale Lemieux 1001
BBa_K3026012 Reporter Toehold norovirus ORF2 VP1 Pascale Lemieux 1001
BBa_K3026013 Reporter Toehold measles virus MG912594.1 Pascale Lemieux 1001
BBa_K3026014 Reporter Toehold measles virus MG912594.1 Pascale Lemieux 1001
BBa_K3026015 Reporter Toehold measles virus MG912594.1 Pascale Lemieux 1001
BBa_K3026016 Reporter Toehold chickenpox virus BAC44882.1 Pascale Lemieux 1001
BBa_K3026017 Reporter Toehold chickenpox virus BAC44882.1 Pascale Lemieux 1001
BBa_K3026018 Reporter Toehold chickenpox virus BAC44882.1 Pascale Lemieux 1001
igem@bcm.ulaval.ca