PARTS
CEAKER
In this project, CEAKER is a synthetically combined receptor used as the diagnostic tool system. TGFBR1, which is originally found in human body, directly interacts with Carcinoembryonic Antigen (CEA). CEA itself functions
as an intercellular adhesion molecule and is upregulated in a wide variety of human cancers[1], including lung cancer. CEA-binding domain mainly lodged in TGFBR1’s extracellular environment. Hence, the domain, boarden between 1th-126th
amino acid, was chosen from TGFBR1.
Furthermore, Transcriptional Coactivator with PDZ-binding motif (TAZ) consists of Tar receptor, Tar HAMP domain, and EnvZ histidine kinase domain. It presents as an oncogene
that is used for the early detection of lung cancer[2]. Therefore, our team selected Taz domains involving 1st-40th and 167th-484th amino acid as part of CEAKER.
Figure 1. Detailed Data for CEAKER PCR Colony Primers.
Biobrick presence inside E. coli shows that PCR colony is essential for multiplications of the whole gene. PCR colony uses either specific primers, i.e. forward primer and reverse primer. Forward primer is
used for elongation of the ribbon from the front or upper chain, while the reverse primer is the opposite.
It is utilized from behind the elongation or bottom chain, for that reason it would produce completed double strands.
PCR colony primer can integrate or connect between one fragment to another fragment of our part. CEAKER PCR colony primers is constructed by using NCBI primer BLAST website. The sequences are served as follows.
Table 1. Detailed Data for CEAKER PCR Colony Primers.