Team:SoundBio/Experiments
Experiments
Protocols and Experiments
Bacterial Growth and Light Exposure ProtocolCreating Yamanaka Agar and Streaking It with K. rhaeticus
DNA Fragment Sample Preparation for Genewiz Sanger Sequencing
iGEM Distribution Kit Plate Instruction
K. rhaeticus HS Media and Culturing
LB Media Recipe
Ligation Protocol with T4 DNA Ligase (M0202)
Making and Running an Analytical Gel Electrophoresis
Making K. rhaeticus Stock from Cryostock
Making HS and Culturing Media
Measuring dsDNA Concentration using the Nanodrop
Miniprep Plasmid DNA Purification from E. Coli
PBS Solution
PCR Amplification of Plasmid DNA (Phusion)
PCR Purification using Silica-Membrane-Based Column Purification
Preparing Electrocompetent K. rhaeticus Cells
Preparing Glycerol Stocks of K. rhaeticus
Preparation of TSS Competent Cells
Preparation of TSS Solution
Qiagen Gel Extraction
Single-temperature Double Digest
SOB and SOC Media Recipe
Storing Organisms in the -80C Freezer
Transformation of K. rhaeticus via Electroporation
TSS Chemical Transformation
-80 Preculture Aliquot Protocol