Team:Northwestern/Experiments2

Northwestern Template

Northwestern Template

EXPERIMENTS

What We Aimed For


Annealing

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Dpn1 Digestion

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Gel Electrophoresis

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Gibson Assembly

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Culture Inoculation

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Ligation

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Making Media

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Miniprep

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PCR

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PCR Cleanup

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Phosphorylation

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Sequencing

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Transformation

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Experiments

Describe the research, experiments, and protocols you used in your iGEM project. These should be detailed enough for another team to repeat your experiments.

A processed gel showing confirmed successful PCR of GFP and UvrA promoter




A processed gel showing confirmed successful PCR of plasmid backbone



Plate with 5 green colonies

The green colonies signify that our Gibson assembly worked, however, the fact that they are green with minimal UV damage indicates that UvrA regulation is not tight. This could be due to the promoter being leaky.

Please remember to put all characterization and measurement data for your parts on the corresponding Registry part pages.

Documentation of the Development of Our Project