Team:NCHU Taichung/Demonstrate

Engineering E.coli

To developed a more efficiency pathway to DMS production, we have expressed related genes in our engineered E. coli, and make sure it successfully carried genes by gel electrophoresis and DNA sequencing. Please see more information here.

Anaerobic culture

We incubated the bacteria in 30℃, 200 rpm with M9 medium in anaerobic bottle.

We observed that the bacteria carrying the genes having ability to fix carbon could have higher maximum absorbance, although they growed more slowly.

Aerobic culture

We incubated the bacteria respectively with M9 medium and LB medium, and in 30℃, 200 rpm.

We observed that the bacteria carrying the pGETS118-LSP plasmid had lower maximum absorbance.

In addition, the bacteria incubated with LB medium growed better than which are incubated with M9 medium.

Culture algae

By designing a series of experiments to find out the growth curve of algae and try to stabilize and optimize the growth conditions, we can understand how much DMS is produced at different stages of the life cycle. Please see more information here.

Model

We have known the mechanism of cloud formation and could produce DMS, the precursor of CCN. We used the model to predict the generation of CNN from DMS.Further speculate on how much CCN will be produced by our E coli.

DMS detection

We used gas chromatography-mass spectrometry to detect and confirm that DMS did exist in the bacterial liquid. Therefore, it verify that our constructed genes can let E.coli successfully produced DMS.