Team:Munich/workspace/Notebook

Team:Munich/workspace/Notebook

April 2019

May 2019

June 2019

July 2019

August 2019

September 2019

October 2019

April 2019

May 2019

June 2019

July 2019

August 2019

September 2019

October 2019

April 2019

May 2019

June 2019

July 2019

August 2019

September 2019

October 2019

April 2019

May 2019

June 2019

July 2019

August 2019

September 2019

October 2019

Alive

Notebook

Assay-Team

Monday	Tuesday	Wednesday	Thursday	Friday	Saturday	Sunday
		1 medium exchange	2 exosome precipitation	3 exosome precipitation	4	5
6	7	8 SDS-PAGE (exosomes)	9 silver staining	10	11	12
13	14	15 DLS	16 exosome precipitation, SDS-PAGE (exosomes)	17 exosome precipitation, SDS-PAGE (exosomes)	18	19
20 DLS	21	22	23	24 RNA isolation exosomes (cells)	25	26
27 seeding	28 RNA isolation	29 exosome precipitation	30 exosome precipitation	31 SDS-PAGE

Monday	Tuesday	Wednesday	Thursday	Friday	Saturday	Sunday
					1	2
3 seeding	4 transfection	5 harvest	6 exosome precipitation	7 exosome precipitation, SDS-PAGE	8	9
10	11 DLS	12	13 His-purification Exosomes	14 SDS-PAGE	15	16
17	18	19 in vitro transcription	20	21 RNA isolation, cDNA synthesis, qPCR	22	23
24	25 RNA isolation (RNeasy Mini kit vs. Trizol)	26 qPCR, Western Blot	27	28	29	30

Monday	Tuesday	Wednesday	Thursday	Friday	Saturday	Sunday
1	2 PCR, agarose gel	3	4 His-Purification Exosomes & VLP, Lysis VLP & Exosomes, RNA isolation, Phenol-/Chloroform extraction	5	6	7
8 DNA digestion	9 reverse transcription	10	11 agarose gel	12	13	14
15	16 Western Blot; DNAse treatment VLPs	17 SDS-PAGE; Western Blot	18 Western Blot	19 non-reducing and native PAGE	20	21 SDS-PAGE
22 reverse transcription	23 qPCR	24 agarose gel	25 reverse transcription	26 Western Blot	27	28
29 Western Blot native; Exosome preciptiation	30 Exosome precipitation, RNA isolation, Western Blot	31 Western Blot

Monday	Tuesday	Wednesday	Thursday	Friday	Saturday	Sunday
			1 Western Blot	2 Western Blot; RNA isolation VLPs	3	4
5 non-reducing and reducing PAGE	6 Lysis of VLP samples, DNAse treatment, RNA isolation	7 reverse transcription, agarose gel	8 Exosome preciptiation, RNA extraction, qPCR Exosomes and VLPs	9 Exosome precipitation, RNA extraction, agarose gel	10	11
12 Western Blot	13 Western Blot	14 VLPs RNA isolation (Trizol)	15 Exosomes RNA isolation (Trizol)	16 SDS-PAGE; Western Blot; VLPs Trizol	17	18
19 SDS-PAGE; Western Blot; reverse transcription of Exosomes	20 PCR; Western Blot; reverse transcription of Exosomes	21 Western Blot; reverse transcription of Exosomes; Dpnr1; qPCR	22	23 qPCR VLPs and Exosomes; Western Blot	24	25
26 RNA isolation	27 Western Blot; reverse transcription of VLPs; VLPs RNA isolation	28 reverse transcription Exosomes and VLPs; Western Blot; RNA isolation Exosomes; qPCR	29 Western Blot	30 qPCR Exosomes and VLPs	31

Monday	Tuesday	Wednesday	Thursday	Friday	Saturday	Sunday
						1
2 rRNA isolation	3 DNase treatment; RNA isolation Trizol; reverse transcription	4	5 WSDS-PAGE; Western Blot	6	7	8
9	10	11	12	13	14	15
16	17	18	19	20	21	22
23	24	25	26	27	28	29
30

Bioinformatics-Team

Monday	Tuesday	Wednesday	Thursday	Friday	Saturday	Sunday
					1	2
3	4	5 cloning V20, V21, V22, V23, V24	6	7	8	9
10	11 cloning V20, V21, V22, V23, V24	12 cloning V20, V21, V22, V23, V24	13 cloning V20, V21, V22, V23, V24	14 cloning V20, V21, V22, V23, V24	15	16
17 cloning V20, V21, V22, V23, V24	18	19	20	21 cloning V20, V21, V22, V23, V24	22	23
24	25	26	27	28	29	30

Monday	Tuesday	Wednesday	Thursday	Friday	Saturday	Sunday
1	2 cloning V20, V21, V22, V23, V24	3 cloning V20, V21, V22, V23, V24	4	5	6	7
8	9 cloning V20	10 cloning V20	11	12	13	14
15 cloning V20, V21, V22, V23, V24; seeding	16	17 transfection	18 cloning B8, B9, B10; fluc-assay	19	20	21
22	23 transfection	24 cloning B8, B9, B10	25 medium exchange	26 cloning B8, B9, B10, B6	27	28
29 cloning B8, B9, B10	30 cloning B8, B9, B10	31

Monday	Tuesday	Wednesday	Thursday	Friday	Saturday	Sunday
			1	2	3	4
5	6 cloning B8, B9, B10, B6	7 cloning B6	8	9	10	11
12	13	14	15	16	17	18
19 cloning B6	20 transfection	21	22 cloning B6; fluc-assay	23	24	25
26 cloning B6	27 cloning B6	28	29	30	31

Monday	Tuesday	Wednesday	Thursday	Friday	Saturday	Sunday
						1
2	3	4 cloning B7	5 cloning B7	6 cloning B7	7	8
9 cloning B7	10 cloning B7	11 cloning B7	12	13	14	15
16	17	18	19	20	21	22
23	24	25	26	27	28	29
30

Exosome-Team

Monday	Tuesday	Wednesday	Thursday	Friday	Saturday	Sunday
		1 medium exchange	2 exosome precipitation	3 seeding	4	5
6 seeding	7 transfection booster blasmid	8 harvesting	9	10	11	12
13 seeding	14 transfection booster plasmid	15 medium exchange	16 harvesting	17 splitting	18	19
20	21	22 cloning	23 cloning	24 cloning	25	26
27 cloning	28	29	30	31

Monday	Tuesday	Wednesday	Thursday	Friday	Saturday	Sunday
					1	2
3 cloning E10, E11; seeding	4 cloning E10, E11; transfectin E6 + E12	5 cloning E13	6 cloning E13	7 cloning E13	8	9
10 seeding	11 transfection	12 cloning E10, E11	13 cloning E10, E11	14 cloning E10, E11	15	16
17	18 seeding	19 seeding	20 transfection for HiBit + qPCR	21 HiBit	22 splitting	23
24 splitting	25 cloning E9/V15	26	27 splitting	28	29	30

Monday	Tuesday	Wednesday	Thursday	Friday	Saturday	Sunday
1 seeding	2 transfection, seeding	3 transfection	4 lysis screening	5 HiBit	6	7
8 seeding	9 transfection (reuptake assay)	10 reuptake assay (medium transfer)	11 reuptake assay	12 seeding	13 transfection (His-purification and reuptake assay)	14
15	16	17	18	19	20	21
22 splitting	23 cloning	24	25	26 seeding, transfecting origin cells	27	28
29	30	31

Monday	Tuesday	Wednesday	Thursday	Friday	Saturday	Sunday
			1	2	3	4
5 seeding	6 cloning E16, transfection (viability assay, qPCR)	7 cloning E16	8 cloning E16; viability assay (trypan blue)	9	10	11
12 seeding	13 cloning: E17; transfection (qPCR, purification)	14	15 reuptake assay	16	17	18
19 reuptake assay (medium transfer); seeding; cloning	20	21	22	23	24	25
26 seeding	27 transfection	28 cytotoxicity LDH assay	29 cytotoxicity LDH assay (medium transfer)	30 cytotoxicity LDH assay	31

Monday	Tuesday	Wednesday	Thursday	Friday	Saturday	Sunday
						1
2 seeding	3 transfection (cytotoxicity LDH assay)	4 seeding recipient cells (cytotoxicity LDH assay)	5 cytotoxicity LDH assay	6 cytotoxicity LDH assay	7	8
9 cloning	10	11 cytotoxicity LDH assay	12 EVOS	13 EVOS	14 EVOS	15
16 seeding	17 transfection (cytotoxicity LDH assay)	18 cytotoxicity LDH assay	19 cytotoxicity LDH assay	20 cytotoxicity LDH assay	21	22
23	24	25	26	27	28	29
30

VLP-Team

Monday	Tuesday	Wednesday	Thursday	Friday	Saturday	Sunday
						1
2	3	4	5	6	7	8
9	10	11	12	13	14	15
16	17	18	19	20	21	22
23	24	25	26	27	28	29
30

Monday

Tuesday

Wednesday

Thursday

Friday

Saturday

Sunday

30
test PEI conditions