Team:ETH Zurich/core/notebook

Notebook

Lab Notebooks

Below you can find the lab notebooks for each of the methods from our genome editing toolbox. To help you find the information you need and reproduce our results we have compiled shortened versions which contain the main experiments under working conditions.

         <a class="pdf-link" href="https://static.igem.org/mediawiki/2019/a/a3/T--ETH_Zurich--invitro-labbook-short.pdf"><img src="" alt="pdf" class="pdf-image">

In Vitro Lab Notebook
         </a>

         <a class="pdf-link" href="https://static.igem.org/mediawiki/2019/1/13/T--ETH_Zurich--recombineering-labbook.pdf"><img src="" alt="pdf" class="pdf-image">

Recombineering Lab Notebook
         </a>

         <a class="pdf-link" href="https://static.igem.org/mediawiki/2019/b/bd/T--ETH_Zurich--yeast-labbook-short.pdf"><img src="" alt="pdf" class="pdf-image">

Yeast Lab Notebook
         </a>

         <a class="pdf-link" href="https://static.igem.org/mediawiki/2019/2/28/T--ETH_Zurich--protein-labbook-short.pdf"><img src="" alt="pdf" class="pdf-image">

Protein Purification Notebook
         </a>

The following notebooks are the full (messy) versions.

         <a class="pdf-link" href="https://static.igem.org/mediawiki/2019/c/c2/T--ETH_Zurich--invitro-labbook.pdf"><img src="" alt="pdf" class="pdf-image">

In Vitro Full Notebook
         </a>

         <a class="pdf-link" href="https://static.igem.org/mediawiki/2019/1/13/T--ETH_Zurich--recombineering-labbook.pdf"><img src="" alt="pdf" class="pdf-image">

Recombineering Full Notebook
         </a>

         <a class="pdf-link" href="https://static.igem.org/mediawiki/2019/6/61/T--ETH_Zurich--yeast-labbook.pdf"><img src="" alt="pdf" class="pdf-image">

Yeast Full Notebook
         </a>

Scroll to see our protocols and primers

Protocols

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         <a class="nav-item nav-link active" id="nav-invitro-protocols-tab" data-toggle="tab" href="#nav-invitro-protocols" role="tab" aria-controls="nav-invitro-protocols" aria-selected="true">In Vitro
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         <a class="nav-item nav-link" id="nav-recombineering-protocols-tab" data-toggle="tab" href="#nav-recombineering-protocols" role="tab" aria-controls="nav-recombineering-protocols" aria-selected="false">Recombineering
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         <a class="nav-item nav-link" id="nav-yeast-protocols-tab" data-toggle="tab" href="#nav-yeast-protocols" role="tab" aria-controls="nav-yeast-protocols" aria-selected="false">Yeast
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               <a class="pdf-link" href="https://static.igem.org/mediawiki/2019/5/53/T--ETH_Zurich--protocol-phage-dna-isolation.pdf"><img src="" alt="pdf" class="pdf-image">

Phage DNA Isolation</a>

               <a class="pdf-link" href="https://static.igem.org/mediawiki/2019/a/a8/T--ETH_Zurich--protocol-dna-extraction-precipitation.pdf"><img src="" alt="pdf" class="pdf-image">

DNA Extraction and Precipitation
               </a>

               <a class="pdf-link" href="https://static.igem.org/mediawiki/2019/a/a2/T--ETH_Zurich--protocol-gel-purification.pdf"><img src="" alt="pdf" class="pdf-image">

Gel Quantification
               </a>

               <a class="pdf-link" href="https://static.igem.org/mediawiki/2019/b/b0/T--ETH_Zurich--phage-amplification-protocol.pdf"><img src="" alt="pdf" class="pdf-image">

Phage Amplification</a>

               <a class="pdf-link" href="https://static.igem.org/mediawiki/2019/8/8d/T--ETH_Zurich--spot-plaque-assay-protocol.pdf"><img src="" alt="pdf" class="pdf-image">

Spot and Plaque Assay
               </a>

               <a class="pdf-link" href="https://static.igem.org/mediawiki/2019/f/f8/T--ETH_Zurich--PTDS-protocol.pdf"><img src="" alt="pdf" class="pdf-image">

PTDS (Library Creation)
               </a>

               <a class="pdf-link" href="https://static.igem.org/mediawiki/2019/9/91/T--ETH_Zurich--txtl-protocol.pdf"><img src="" alt="pdf" class="pdf-image">

TXTL Expression</a>

               <a class="pdf-link" href="https://static.igem.org/mediawiki/2019/2/21/T--ETH_Zurich--protocol-phage-concentration.pdf"><img src="" alt="pdf" class="pdf-image">

Phage concentration
               </a>

               <a class="pdf-link" href="https://static.igem.org/mediawiki/2019/6/6f/T--ETH_Zurich--protocol-qPCR.pdf"><img src="" alt="pdf" class="pdf-image">

qPCR
               </a>

               <a class="pdf-link" href="https://static.igem.org/mediawiki/2019/9/94/T--ETH_Zurich--protocol-Recombination.pdf"><img src="" alt="pdf" class="pdf-image">

Recombination
               </a>

               <a class="pdf-link" href="https://static.igem.org/mediawiki/2019/a/af/T--ETH_Zurich--protocol-bioreactor-operation.pdf"><img src="" alt="pdf" class="pdf-image">

Library Evolution with Bioreactor</a>

               <a class="pdf-link" href="https://static.igem.org/mediawiki/2019/3/32/T--ETH_Zurich--electroporation-protocol.pdf"><img src="" alt="pdf" class="pdf-image">

Electroporation</a>

               <a class="pdf-link" href="https://static.igem.org/mediawiki/2019/d/df/T--ETH_Zurich--yeast-transformation-protocol.pdf"><img src="" alt="pdf" class="pdf-image">

Yeast Transformation
               </a>

               <a class="pdf-link" href="https://static.igem.org/mediawiki/2019/a/a4/T--ETH_Zurich--yeast-extraction-protocol.pdf"><img src="" alt="pdf" class="pdf-image">

Yeast Plasmid Extraction
               </a>

               <a class="pdf-link" href="https://static.igem.org/mediawiki/2019/b/b6/T--ETH_Zurich--NAT-PCR-protocol.pdf"><img src="" alt="pdf" class="pdf-image">

NatMX Cassette PCR
               </a>

               <a class="pdf-link" href="https://static.igem.org/mediawiki/2019/2/20/T--ETH_Zurich--crispr-yeast-protocol.pdf"><img src="" alt="pdf" class="pdf-image">

CRISPR Protocol
               </a>

               <a class="pdf-link" href="https://static.igem.org/mediawiki/2019/9/9f/T--ETH_Zurich--cleanup-yeast-protocol.pdf"><img src="" alt="pdf" class="pdf-image">

DNA Extraction Cleanup
               </a>

               <a class="pdf-link" href="https://static.igem.org/mediawiki/2019/b/bc/T--ETH_Zurich--colony-PCR-yeast-protocol.pdf"><img src="" alt="pdf" class="pdf-image">

Yeast Colony PCR
               </a>

Primers

     <a class="pdf-link" href="https://static.igem.org/mediawiki/2019/d/d5/T--ETH_Zurich--primers.pdf"><img src="" alt="pdf" class="pdf-image">

Primer List
     </a>