Team:CSU Fort Collins/Protocols
Find all of our protocol and lab notes on our team's Benchling Page .
Here is a summarized list of procedures and experiments iGEM has run for the project:
- Heat-shock transformation of NEB 5-Alpha E. coli cells
- Making and sterilizing various selective media types
- Restriction digestion on plasmid fragments to analyze construct accuracy and completeness
- Measurement of fluorescence produced by plasmid constructs using a fluorometer
- Making competent E. coli cells
- 0.8-1% gel electrophoresis on plasmids and other constructs
- PCR of gene inserts into a plasmid to ensure accuracy of gene ligation using PuRETaq beads
- PCR reagent optimization experiments
- Miniprep plasmid/construct isolation and concentration
- Designing primers for specific sequences using online programs
- Restriction digestion and ligation of genes into plasmid vectors
- Using a Fisher Scientific Nanodrop One to measure purity and concentration of isolated DNA’s
- Analyzing microbial growth using a spectrophotometer
- Induction of prophage using Mitomycin C
- Quantifying phage concentration using phage plaque assays