Team:BIT/Team/Notebook

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  • We use two weeks to build our team and brainstorming the project.
    We held a lecture on knowledge for high school students and led them to the lab.

  • Determine the best project in the brainstorming by PI Lu Xuefei.
    Confirm the project and the team members are divided into three groups.
    The literature research is carried out to make up the details.

  • Continue to conduct literature research to complement the project.
    Planning and implementation of HP activities, preparing for educational activities for university students.

  • Hosting field events and science lectures for college students.
    Issue questionnaires to investigate college students' views on our projects.

  • Continue to investigate.

  • Opened our own Bilibili account and launched promotional videos.
    Continue to investigate.

  • Conducted laboratory safety training and conducted the most basic molecular cloning experiment training.

  • Making microfluidic chip production learning.
    Continue to investigate.

  • Start the preparation of the experimental protocol and prepare the reagents.
    Continue to make microfluidic chip production learning.
    Continue to investigate.
    Going to the China Academy of Environmental Sciences for an interview.

  • Students who have been recruited for art design and market research have been recruited.
    Continue to conduct experimental training on experimental skills.
    Continue to learn the production of microfluidic chips.
    I went to the 8th team of the Public Security Fire Brigade of Shijingshan District in Beijing for an interview.

  • Initial preparation of the experimental protocol and preparation of the missing reagents.
    Create a new version of the BIT team molecular clone manual to guide the experimental process.

  • Conduct a preliminary experiment.
    Preliminary production of microfluidic chips.
    Design hardware part.

  • Production of PDMS chips.
    Completed the preliminary design of the hardware.

  • Determined the genetic line and eventually formed an experimental plan.

  • Begin experimentation, perform PCR experiments and construct plasmids with five promoters that bind to GFP.
    Learning and making PMMA chips.
    Designing hardware devices.

  • Continue to build plasmid construction and extraction.
    Redesigned and purchased the primers required for PCR.
    Exchanged with the BIT_China team on the progress of the project and the problems encountere.

  • Re-validated the constructed plasmid.
    Fabrication of PMMA chips.
    Communicate with ASTWS-CHINA team about new media promotion.

  • Re-implement PCR experiments and plasmid construction.
    Perform PMA chip effect detection.

  • Two sets of promoter + GFP plasmids were constructed and plasmid transformation was constructed to construct strains.

  • The DNA damaging agent was added to effect the effect of detecting the strain of the umuDC promoter, and it was confirmed that the promoter did not meet the requirements.

  • 3D printed the first version of the instrument and purchased hardware such as temperature control components.
    Add DNA damaging agent to detect the effect of dinD promoter, confirm that the promoter can not meet the requirements.

  • Continue to build the strain.
    Participated in CCIC and received suggestions for improvement projects.
    Conducted communication with the team of Shenzhen University and proposed suggestions for each other's projects.
    Exchanged with micro-fluidic chips with Zhejiang University.
    Continue to build the instrument.

  • Rethinking and confirming the new project idea, and improving the experimental plan.
    Continue to repeat the experiment.
    Feedback from previous survey participants.

  • A plasmid containing "recA+GFP" was constructed and plasmid transformation was performed to construct a strain.

  • Add DNA and H2O2 DNA damaging agents to detect the effect of the recA promoter, confirming that the promoter meets the requirements.

  • More types of DNA damaging agents and non-DNA damaging agents are added to detect the characterization of the gene lines performed.

  • Validation of strain activity using the part mentioned in the Silver Award standard.
    Building WIKI.

  • Continue to make chip fabrication and device construction; continue to characterize gene lines.
    Building WIKI.

  • Complete the entire project.
    Building WIKI.
    Waiting wiki freeze.

  • Build the wiki
    upload the part experience prepare the medals 10.20 wiki freeze!