Team:BIT/Description

Project Inspiration and Description

Oxidative stress damage is a phenomenon in which an organism suffers from extreme conditions such as toxic substances and radiation in the external environment, causing DNA mutation or even DNA strands breaks. Since oxidative stress damage causes changes to DNA, it would probably cause cancer or other severe illness.Since most people are unaware of the oxidative stress damage to their bodies, and cannot get valid treatment in time, thus cause illness. Also in the current situation, there is no reading instrument that can be used in everyday life, which can be carried by ordinary people.

We use DNA damage repair mechanism as a basic for constructing an oxidative stress damage microbial sensor. For realization, we plan to get promoter sensitive to oxidative stress damage by promoter trapping, then construct a bio-amplification system, also include the green fluorescent protein report gene. And by detecting the intensity of green fluorescence to characterize the signals of oxidative stress damage.

In terms of hardware, to enable portable and real-time monitoring, we integrate basic operation units such as sample reaction, separation, cell culture, and detection involved in the fields of chemistry and biology into a small chip to meet our needs of less-sample consumption and portability. We will carry the temperature control module and the fluorescence detection system at the same time. Build a portable fluorescence detection device and achieve the goal of portable detection.

Our project provides the public with a portable oxidative stress damage detection instrument that allows people to obtain data on their own oxidative stress damage in real-time and can achieve active monitoring of the surrounding environment. This will avoid the risk of getting illness of the unaware patients due to long-term injury, it will remind patients who have been affected to seek medical attention as soon as possible.

Reference:

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