Team:Auburn Alabama/Notebook
Notebook
January 18th - first meeting to discuss our possible project idea -we decided to focus on detecting possible harmful substances in makeup -we assigned a possible substance for us to detect to each member to do further research on -lead- Sarah, aluminum- Chase, sulfate- Andrew, paraben- Amy and Jaeyoung, Phtalates- Michael, Triclosan – Scott January 24, 2019- second meeting January 28th- looked at Avon laboratories, ARKRAY inc, and Siemens healthcare as practitioners of cell transformation stuff and synthetic biology (Amy) February 19th – researched into cell free protein synthesis March 8th – gave everyone an excerpt from the Genetic Engineering Book to read (pages 24-37) March 18th – planned next weeks practice transformation experiment with kit KIT: 1x DNA Playground 1x Zero to Genetic Engineering Hero book 4x Canvas Kits 1x Group Engineer-it Kit (enough for 8 genetic engineering experiments - you can sub in your own DNA plasmids) 2x Plate Extract-it Kits4 March 22nd- pour plates March 26th- practice transformation procedure with kit March 27th- checked plates- two worked two failed April 2nd- looked into other teams projects (“Here are a few of last year’s high school teams and their projects: Bioremediation of biofilm https://2018.igem.org/Team:US_AFRL_CarrollHS Inexpensive diagnostics kit to detect AND predict cholera outbreaks https://2018.igem.org/Team:Lambert_GA Visual detection of excess fluoride in water https://2018.igem.org/Team:East_Chapel_Hill HIV-1 diagnostics kits for infants in developing nations https://2018.igem.org/Team:Austin_LASA Arsenic detector https://2018.igem.org/Team:Tacoma_RAINmakers Getting E. coli to light up LIGHTBULBS https://2018.igem.org/Team:Pittsburgh_CSL Trying to cure Huntington’s disease https://2018.igem.org/Team:New_York_City") April 5th- discussed the other teams projects and what we could realistically do April 9th- discussed our wiki and created bios, gave Alice pictures for our IGEM avatars -looked into metal sensitive promoters that we could use in our plasmid, we were each supposed to look one up for the next meeting (in stock in bio brick registry) April 16th- we talked about the promoters we found and now we were each supposed to find a reporter protein in the bio brick registry (in stock) April 23rd – discussed reporters we found + Cowen Erwin joins April 26th- discussed picking our RBS and whether we were going to focus on cobalt or mercury promoter; we decided on the cobalt promoter because it also could detect nickel but the team had not characterized it on the IGEM wiki April 30th- Began planning our summer and what we were going to do May 3rd- We discussed how we would do the transformation experiment with our individual parts (this was before we knew we could send in the plasmid pairs to companies to have them synthesized for us) Also figured out that the RBS came with the kits IGEM sends us) May 10th- Our logo is finished by Alice May 30th- we discussed our summer plans and when we were going to do the transformation- mainly discussed schedules June 5th- Discussed our transformation and finalized our schedules for the summer June 7th- delegated duties to team members for the short term -1. Safety form -2.project description and inspiration (Andrew and Michael) -3. IGEM wiki- Chase and Alice -4. Human practices- Amy, Cady, Cowen, Jae -5. Safety- Scott June 11th- Skype with University of Alabama team -rediscussed the order and method of our transformation and re-discussed our plans, talked about our progress June 19th- was assigned our mentor June 24th - Sarah completed the draft of the Safety Form, and Michael and Andrew completed the Project Description and Inspiration to our wiki. July 1st - found out that plasmid construction is very hard as you lose yield in every step; so made IDT account and started designing our plasmid. July 22nd -ordered fully constructed plasmids from IDT August 15th - received the plasmids, one with chromoprotein and another with fluorescnce that are sequence verified, that we ordered. Members gathered and discussed about transformation and characterizing. September 1st - ordered nickel acetate powder September 3rd - got our plasmids but we didn’t have IDTE buffer to resuspend them so planned to use distilled water for our transformation. September 9th - Sarah worked on our wiki’s abstract. September 11st– first trial for transformation September 18th - found out that transformation did not work, so started second trial for transformation September 19th - found out that transformation did not work, so started third trial for transformation September 20th - found out that transformation did work September 30th - tried testing with nickel solution to see if the transformation worked to change color. Found out that the color did not change. October 5th – Tried to test again in nickel October 6th - found out that the gene may be mutated? October 12th - meeting at the public library to grind out wiki -we assigned different sections of wiki pages to do -Sarah – Project Design, Safety -Scott – Safety, Notebook -Michael - Notebook