Team:AHUT China/index

AHUT-iGEM Top

"We made prelimilary progress in the study of CO2 capture by establishing a mutated carbonic anhydrase last year, and found that the stability and enzyme activity of the mutated carbonic anhydrase were significantly increased, but no further research and development was carried out. This year, on the basis of last year, we will modify the mutated carbonic anhydrase gene to obtain genetically engineered expression bacteria that express the carbonic anhydrase with aldehyde on the protein surface under the reaction of formylglycine-generating enzyme, which in turn binds to the amino group of the immobilized carrier. The immobilization of carbonic anhydrase is realized to improve its stability, facilitate its repeated use, and achieve industrial application."

— ABOUT OUR PROJECT —

Project Inspiration

In recent years, the application of carbonic anhydrase in CO2 capture has attracted widespread attention and has become an efficient and environmentally friendly strategy.

Carbonic Anhydrase

Carbonic Anhydrase is a zinc-containing metalloenzyme. When the Zn2+ ion abstracts a proton from a surrounding water molecule, creating a hydroxide ion that is negatively charged and can attack the partially positive carbon atom on a solubilised CO2 molecule to form a HCO3- ion, the hydration of CO2 occurs.

The Enzyme Fixed

Formylglycine generating can selectively recognize and oxidize the cysteine residues in the sulfatase subunit of the protein terminal to form formylglycine (FGly) residues containing aldehyde groups.

Gold

Registration and Giant Jamboree Attendance The AHUT_China team has been successfully registered on March 1, 2019,and the team will take part in giant Jamboree . Competition Deliverables Our various achievements will be presented here. Wiki Poster Presentation Judging Form Attributions The hard work of everyone constitutes the success of the whole team. In attribution you can look at each person's contribution to the team. Project Inspiration and Description What inspired us to start and finish our project? Click on inspiration and description. Characterization / Contribution BBa_K2762004 was characterized and enzyme activity data were added. Detailed data is looked in the Characterization.

Silver

Validated Part / Validated Contribution We created a new part ,for enzyme fixation. Click on new part to view the detail information. Collaboration We have conducted friendly cooperation with ocean university of China, Universitat Potsdam, Germany. Detail information will be presented in Collaboration. Human Practices We promote iGEM and synthetic biology to people of different ages. Read more activities in HP For Silver.

Bronze

Registration and Giant Jamboree Attendance The AHUT_China team has been successfully registered on March 1, 2019,and the team will take part in giant Jamboree . Competition Deliverables Our various achievements will be presented here. Wiki Poster Presentation Judging Form Attributions The hard work of everyone constitutes the success of the whole team. In attribution you can look at each person's contribution to the team. Project Inspiration and Description What inspired us to start and finish our project? Click on inspiration and description. Characterization / Contribution BBa_K2762004 was characterized and enzyme activity data were added. Detailed data is looked in the Characterization.

Team Members

These people make our project great!