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  • Team:Jiangsu High School/Experiments
    • FBS <br /> ...ed to prevent ice crystal growth during the freezing of the cells), 10% of FBS (fetal bovine serum, a blood fraction that remains after coagulation of blo
    50 KB (7,290 words) - 20:30, 21 October 2019
  • Team:QHFZ-China/Demonstrate
    ...vine serum was added to 1000 μL final reaction system. When the volume of FBS fraction was 1/1000, the UA detection efficacy was unaffected by serum (Fig ...ent of dsRed fluorescence / OD600 after 20 μM UA addition with or without FBS. Data were shown as mean + SD. N = 3 technical repetitions.
    24 KB (3,615 words) - 07:02, 18 November 2019
  • Team:XJTLU-CHINA/Experiments
    ...al neurons and glial cells. All of the Neuro-2a cells were incubated in 5% FBS-added DMEM medium with anti-anti in a 24-well plate until the cells were gr ...we changed the medium to phenol-free RPMI1640 medium with 5% exosome-free FBS, and additional glutamate were was added to ensure that the concentration o
    53 KB (6,741 words) - 04:54, 13 December 2019
  • Team:XHD-WS-Wuhan-A/experiment/notebook/
    ...sinize GES1 and SGC-7901 for 1 minute, then remove the trypsin. Add 10% of FBS 1640 &nbsp;&nbsp;&nbsp;&nbsp; growth medium, and use the pipette to stir to ...g condition<span style="font-family:宋体">：</span>RPMI1640(w/oHepes)10%FBS
    23 KB (3,046 words) - 17:59, 21 October 2019
  • Team:NJTech China/Experiments
    which is supplemented with FBS 10% and ConA 2.5 µg/ml) containing various concentrations of IL-2 ...incubated for 20 min at 37℃. After washing by RPMI 1640 containing 10%FBS (Gibco, Brazil), cells are
    42 KB (4,838 words) - 18:53, 21 October 2019
  • Team:LZU-CHINA/Results/Protocols
    ...hamber at 37°C with 5% CO2 in media containing DMEM supplemented with 10% FBS, 1 mM sodium pyruvate, 1 unit/ml penicillin, 1 μg/ml streptomycin, 2 mM L- ...cells were washed away with PBS, replaced with fresh serum-free medium/1% FBS medium, and photographed by inverted microscope. Image J analyzes cell craw
    62 KB (6,515 words) - 07:46, 20 October 2019
  • Team:NUDT CHINA/Experiments
    ...e Dulbecco's Modified Eagle Medium( DMEM ) + 10% PAN™ Fetal Bovine Serum(FBS) + 1% Hyclone™ 100X Penicillin-Streptomycin Solution(P/S).</p> ...ee Dulbecco's Modified Eagle Medium( DMEM ) +10% PAN™ Fetal Bovine Serum(FBS)+1%Hyclone 100X Penicillin-Streptomycin Solution(P/S).</p>
    13 KB (1,732 words) - 00:20, 22 October 2019
  • Team:IISER Kolkata/Notebook
    US origin FBS was heat-inactivated, filter sterilized and aliquoted added to the media along with 10% FBS and filter sterilized.
    36 KB (4,152 words) - 02:17, 22 October 2019
  • Team:QHFZ-China/Notebook
    ...complete DMEM (CD) medium (89% High-glucose DMEM, 10% fetal bovine serum (FBS) and 1% Penicillin / streptomycin). Generally, the medium was changed every ...Then the medium was filtered by 0.2 μM filter and conserved at 37℃. 10% FBS was added before use and the final concentration of UA was 1000 μM or 1600
    12 KB (1,732 words) - 07:05, 18 November 2019
  • Team:IISER Tirupati/Protocols
    ...0% FBS, it was essential to filter the spent media before the assay, since FBS contains lactate dehydrogenase enzyme which will cleave the lactate to pyru
    31 KB (4,992 words) - 14:00, 21 October 2019
  • Team:MIT/Human Practices
    ...also advised us to remove all FBS, because starved HL-60s will respond to FBS, which would mask their response to IL-8. Finally, he provided us with more
    13 KB (2,142 words) - 03:54, 22 October 2019
  • Team:SUSTech Shenzhen/Notebook
    1. Prepare DMEM medium containing 10% FBS Mix the filtered 10% FBS with DMEM medium, and preheat the medium to 37 ℃ before use.
    16 KB (2,331 words) - 08:58, 21 October 2019
  • Team:SMMU-China/Notebook
    cells once with 5ml of DMEM (with no FBS). Add 1 ml trypsin-EDTA into the flask. After 1-1.5 minutes, add 5ml of DMEM (with 10% FBS) and resuspend softly. Then transfer the cells into 15ml falcon and centrif
    78 KB (7,504 words) - 12:20, 21 October 2019
  • Team:REC-CHENNAI/Protocol
    ...e&rsquo;s Medium (DMEM) solution supplemented with 10% fetal bovine serum (FBS) and antibiotics.</li> ...Modified Eagle’s Medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 100 U/ml penicillin, and 0.1 g/ml streptomycin.</li>
    37 KB (4,747 words) - 14:33, 20 October 2019
  • Team:Nanjing NFLS/Notebook
    ...the cells one day prior to transfection and switch to antibiotic-free 10% FBS DMEM medium and place 1X105 per well in 24-well plates. According to PGL3-B ...ed cells were transferred to a T75 flask and 9 mL of medium containing 10% FBS was added.</span> </p>
    54 KB (6,919 words) - 17:39, 19 October 2019
  • Team:NUDT CHINA/Contribution
    ...ls were cultured in High Glucose Dulbecco's Modified Eagle Medium with 10% FBS and under condition of 37℃, 5% CO2. Because the Rluc hasn’t 100% expres
    7 KB (821 words) - 03:36, 22 October 2019
  • Team:XJTLU-CHINA/Demonstrate
    ...d changed the DMEM medium to RPMI1640 phenol-free medium (with 5% exo-free FBS, 1% Penicillin and Streptomycin added). To simulate the inflammation-induci
    29 KB (3,293 words) - 04:45, 13 December 2019
  • Team:Shanghai-United/Parts
    ...ri dishes with 10 ml of cell culture media made with 500ml DMEM + 50ml 10%FBS+5.5ml Streptomycin/Penicillin(to avoid growth of bacteria). We used 18 of
    20 KB (2,660 words) - 15:31, 9 December 2019
  • Team:SUSTech Shenzhen/Experiments
    Medium Composition: DMEM Medium + 10% FBS
    11 KB (1,443 words) - 15:50, 20 October 2019
  • Team:Fudan-TSI/Protocols
    ...<p>Cells were cultured in DMEM supplemented with 10% FBS (HyClone), 100 U/ml penicillin, 100 μg/ml streptomycin and 1x GlutaMax (Gi
    19 KB (1,834 words) - 07:09, 29 August 2019

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