Team:Wageningen UR/Parts

Xylencer

Parts

Group Photo

On this page, all our parts are listed. All these parts are uploaded to the iGEM Registry of Standars Biological Parts. We submitted biobricks for the best basic part, the best composite part and the best parts collection.

Part Collection

Table 1: Overview of all parts related to CRISPR-Cas and uploaded as best parts collection.
Biobrick Code Part Name Designer
BBa_K3286000 AcrIIA4 Alex
BBa_K3286001 sgRNA Cas9 + NT Spacer Alex
BBa_K3286002 rho-independent terminator Alex
BBa_K3286003 sgRNA expression cassette (single target) Alex
BBa_K3286004 lac operator Alex
BBa_K3286005 Ptet Alex
BBa_K3286006 bi-directional terminator Alex
BBa_K3286007 Prarthana terminator Alex
BBa_K3286008 IPTG inducible dCas9 expression cassette Alex
BBa_K3286009 AcrIIA4 expression cassette Alex
BBa_K3286010 dCas9-AcrIIA4 gene circuit Alex
BBa_K3286011 sgRNA expression cassette (double target) Alex
BBa_K3286012 dCas9 Alex
BBa_K3286013 RBS C11 Alex
BBa_K3286014 RBS C7 Alex
BBa_K3286015 RBS B10 Alex
BBa_K3286016 RBS D2 Alex
BBa_K3286017 RBS B6 Alex
BBa_K3286018 RBS A12 Alex
BBa_K3286019 RBS A9 Alex
BBa_K3286040 dCpf1 Sebastiaan
BBa_K3286041 CRISPR array Sebastiaan
BBa_K3286042 Lambda PL promoter, OL operators and 5' UTR Sebastiaan
BBa_K3286043 Lambda PR and PRM promoter, OR operators and 5' UTR Sebastiaan
BBa_K3286044 Lambda PL, PRM and PR promoters, operators and 5' UTR's Sebastiaan
BBa_K3286045 Monomeric Red Fluorescent Protein (mRFP) Sebastiaan
BBa_K3286046 mRFP-GFP divergent, Bacteriophage Lambda promoters, operators and 5'UTR's Sebastiaan

Other Parts

Table 2: Overview of all parts related to protein expression and purification.
Biobrick Code Part Name Designer
BBa_K3286100 T7 promoter + BCD system + Strep tag + TEV cleavage site Robert
BBa_K3286101 Strep tag Robert
BBa_K3286102 MBP Robert
BBa_K3286103 Strep tag + MBP + TEV cleavage site Robert
BBa_K3286104 T7 promoter + BCD system + Strep tag + MBP + TEV cleavage site Robert
BBa_K3286105 PD1764 Robert
BBa_K3286106 PD1764sh Robert
BBa_K3286107 HAD domain Robert
BBa_K3286108 Chitin binding domain Chitinase A1 Robert
BBa_K3286109 Chitin binding domain Chitinase A1 sh Robert
BBa_K3286110 3LysM Robert
BBa_K3286111 LysM domain PD1764 Robert
BBa_K3286112 R30 + terminator Robert
BBa_K3286113 T7 promoter + BCD system + Strep tag + TEV cleavage site + PD1764 + terminator Robert
BBa_K3286114 T7 promoter + BCD system + Strep tag + TEV cleavage site + PD1764sh + terminator Robert
BBa_K3286115 T7 promoter + BCD system + Strep tag + TEV cleavage site + HAD domain + terminator Robert
BBa_K3286116 T7 promoter + BCD system + Strep tag + TEV cleavage site + CBD Chitinase A1+ terminator Robert
BBa_K3286117 T7 promoter + BCD system + Strep tag + TEV cleavage site + CBD Chitinase A1 truncated + terminator Robert
BBa_K3286118 T7 promoter + BCD system + Strep tag + TEV cleavage site + 3LysM + terminator Robert
BBa_K3286119 T7 promoter + BCD system + Strep tag + TEV cleavage site + LysM domain PD1764 + terminator Robert
BBa_K3286138 flg22 Cleo
BBa_K3286139 NLP20 Cleo
Table 3: Overview of all parts related to the DSF-sensing circuit.
Biobrick Code Part Name Designer
BBa_K3286200 RpfCch Ben
BBa_K3286201 RpfG Ben
BBa_K3286202 Vc2 Riboswitch Ben
BBa_K3286203 sfGFP Ben
BBa_K3286204 J23100 + RBS0034 + RpfCch + RBS0034 + RpfG + Terminator T0 Ben
BBa_K3286205 J23108 + Vc2 Riboswitch + sfGFP + Terminator T0 Ben
BBa_K3286206 RpfCch sfGFP fusion Ben
BBa_K3286207 RpfCch (TMD) sfGFP fusion Ben
Table 4: Overview of all parts related to the Molecular Timer.
Biobrick Code Part Name Designer
BBa_K3286215 KaiABC Oscilator Alba
BBa_K3286216 KaiABC Oscilator + rhamnose promoter + KaiBC promoter Alba
Table 5: Overview of all parts related to phage engineering.
Biobrick Code Part Name Designer
BBa_K3286264 Lambda gpD without stop codon + translation initiation sequence Marijn
BBa_K3286265 Phage T7 P10 without stop codon + translation initiation sequence + T7 promoter Marijn
BBa_K3286266 Phage Lambda gpD insertion homology region 1 Marijn
BBa_K3286267 Phage Lambda gpD insertion homology region 2 Marijn
BBa_K3286268 CGSGSGSG Linker Marijn
BBa_K3286269 Lambda gpD - Chitinase A Fusion Marijn
BBa_K3286270 Lambda gpD - GFP fusion Marijn
BBa_K3286271 Lambda gpD - HAD fusion Marijn
BBa_K3286272 Lambda gpD - PD1764 fusion Marijn
BBa_K3286273 Phage T7 P10 - Chitinase A fusion Marijn
BBa_K3286274 Phage T7 P10 - GFP fusion Marijn
BBa_K3286275 Phage T7 P10 - HAD fusion Marijn
BBa_K3286276 Phage T7 P10 - PD1764 fusion Marijn
BBa_K3286277 Lambda gpD - Chitinase A recombination module Marijn
BBa_K3286278 Lambda gpD - GFP recombination module Marijn
BBa_K3286279 Lambda gpD - HAD recombination module Marijn
BBa_K3286280 Lambda gpD - PD1764 recombination module Marijn
BBa_K3286281 Ribosomal frameshifting sequence Santi
BBa_K3286282 Lambda gpD - linker - Chitinase A1 Santi
BBa_K3286283 Lambda gpD - ribosomal frameshifting sequence - Chitinase A1 Santi
Table 5: Overview of all parts related to LAMP assays.
Biobrick Code Part Name Designer
BBa_K3286230 LAMP detection of X. fastidiosa Niels