Team:Wageningen UR/Experiments

Xylencer

Protocols

Group Photo

On this page, the protocols used by our team can be found. All protocols are uploaded on protocols.io. Protocols are derived from iGEM, literature and suppliers or are designed by ourselves.

Measurements Nucleotide and Protein Concentration

Qubit
Nanodrop

Agarose gel

Agarose Gel Electrophoresis
DNA Purification Agarose Gel

PCR

Overlap Extension PCR
OneTaq
Phusion
Phire
Q5

Cloning

Chemically Competent Cells
Heat Shock Transformation
Electrocompetent cells
Electroporation
Mix&Go Competent Cells
Ligation
Restriction Enzyme Digestion
Gibson
Golden Gate Assembly
Removal Kanamycin-R gene

RNA Isolation

RNeasy
Trizol
DNase I Treatment

Protein Expression and Purification

Protein Expression
Protein Sample Preparation
Purification Gravity-Column
Purification FPLC
SDS Page Gel
Tricine SDS Page Gel

Chitin Binding

Chitin Binding & Bradford Assay
Chitin Magnetic Beads

Phages

Phage Stock
Phage Genome Isolation
Spot Assay
Plack Assay
Genome Concatermerization
TXTL Phage Production

Phage Repression

Growth Curve Analysis
GFP-RFP Plate Reader Assay
Fluorescent Loss Assay Acr

Yeast

Yeast Competent Cells
Transformation

Molecular Timer

72h OD&GFP measurements

Plants

Plant Infection
Xylem Inoculation
Lesion Size

Insects

Capture
Storage
Transmission Assay

LAMP

LAMP assay
LAMP Paraffin encasing
X. fastidiosa extraction
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