Team:TAU Israel/Experiments

 

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CLONING PROTOCOL

The following file contains the Gibson cloning protocol that we performed in order to clone our different plasmids

PLAQUE ASSAY PROTOCOL

This Plaque assay protocol describes the way to test whether our bacteria kills its target. This protocol was performed 3 times. The first time with PAO1 only, and the other two times for testing our E. coli system as described in the results.

PROTEIN EXPRESSION PROTOCOL

The following file contains the protein expression protocol that we performed in order to verify that we recieved the desired proteins in our system.