Team:AHUT China/Collaborations

Collaborations

We collaborated with two schools that one is at home and the other one is abroad

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Collaboration with OUC

Our vision of a synthetic biology lab

When we were looking for cooperation opportunities, we discovered the cooperation of synthetic biology laboratories published by the OUC team on the iGEM website. The team members agreed that this was a good chance, so we got in touch with the OUC team.

In order to help the OUC team, we looked up a lot of information about synthetic biology laboratories so that we could summarize the experimental equipment that we could recommend to them. We also mapped out a simple layout of the lab, a little bit simple, but a good expression of our idea of the lab.

At 8:00 PM on July 24th, we officially conducted a videoconference with OUC. At the meeting we gave a detailed account of our vision of the laboratory and had a friendly discussion with the other parties.

Because of the friendly communication on the video conference, we were happy to accept the cooperation of the OUC team on the experiment. So this friendly and cooperative relationship continued.

ColWithOUC
Fig.1. After meeting

Measurement and fluorescence

In order to solve the problem of modularization of riboswitches, the team of OUC proposed the theory of modular riboswitches. They use the “Stabilizer” to stabilize the structure of the riboswitch and use the “tunner” to insulate Stabilizer and GOI allowing for predictable tuning.

To test the feasibility of the OUC team's modular ribose switch in practical use, they needed to express it in different experimental environments to explore the stability of the structure.

Fig.2. Help OUC test

So we were delighted to work with ocean university of China to help test whether their project could be equally successful in different laboratories.

The team of ocean university of China sent their samples to us, and we carried out specific operations by referring to their experimental methods. Finally, we fed back our experimental results to help them perfect their project.

feedbackOUC
Fig.3. Feedback for OUC

Detailed experimental methods can be click to view.

EXPERIMENTAL METHODS

and measuring results can be click to view .

MEASURING RESULTS

Collaboration with UP

While browsing the iGEM website, we found University Potsdam, a Germany university seeking cooperation. We are very happy because the content of this cooperation is quite consistent with our project, which is related to the research of thermal stability.

After receiving the emails from the leaders of both sides, we have established a friendly cooperative relationship. We sent the protein sequence of the wild-type Carbonic Anhydrase 2 which comes form our project, and then they used their software to provide a series of heat-resistant mutation sites and predicted temperatures.

We are very grateful to the university for providing us with so many mutation sites as a reference for our project experiment.

Fig.4. Communicate with UP

CATALOGUE

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